Glycogen Phosphorylase BB (GPBB) Antibodies

Glycogen phosphorylase BB (GPBB)

Glycogen phosphorylase BB (GPBB) is another potential candidate for clinical diagnosis of myocardial ischemia within the first 1-3 hours after chest-pain onset. GP is bound to glycogen in the sarcoplasmic reticulum and catalyzes the first step of glycogenolysis after activation, which involves the separation of glucose-1-phosphate from glycogen.

Three GP isoenzymes are present in human tissue and are named according to the tissue of their initial description: GPLL (liver), GPMM (muscle) and GPBB (brain). In addition to its occurrence in brain, GPBB is also found in high concentrations in heart muscle. During myocardial ischemia, activation of GPBB results in an increase in glycogen degradation. Thus, GPBB is released from glycogen and then enters the bloodstream. Initial research has shown higher sensitivity of GPBB within the first 4 h following chest-pain onset in comparison to other cardiac markers. In addition, GPBB seems to indicate necrotic cell damage and, in particular, ischemic processes, e.g., as observed with unstable angina pectoris (UAP). Therefore, GPBB may serve as another candidate marker to provide earlier diagnosis in AMI^1-4.

AvantGen’s anti-GPBB antibody clones are highly specific and show no cross-reactivity to GPLL and GPMM and are suitable for sensitive GPBB assay development.

References
1. Peetz, D., et al.,Glycogen phosphorylase BB in acute coronary syndromes.ClinChem Lab Med, 2005; 43(12): p. 1351-8.

2. Entman, ML., et al.,Association of glycogenolysis with cardiac sarcoplasmic reticulum: II. Effect of glycogen depletion, deoxycholatesolubilization and cardiac ischemia: evidence for a phorphorylasekinase membrane complex.J Mol Cell Cardiol, 1977; 9(7): p. 515-28.

3. Krause, EG.,et al.,Glycogen phosphorylaseisoenzyme BB in diagnosis of myocardial ischaemic injury and infarction.Mol Cell Biochem, 1996; 160-161: p. 289-95.