The rabbit monoclonal antibody advantage

The rabbit immune system generates and affinity matures antibodies by mechanisms that differ from those of mice and other rodents. Antibodies produced by rabbits normally have 10 to 100-fold higher affinity for antigen than mouse monoclonal antibodies. In addition, the rabbit immune system is particularly adept at generating antibodies that are able to distinguish between very similar molecules with subtle structural variations.  Thus, these products are exceptional research antibodies.

In 2015, the NCI awarded a contract to AvantGen to develop rabbit monoclonal antibodies against peptides derived from potential cancer biomarkers. Based on the high quality of the anti-peptide antibody clones that were generated from Phase I projects, AvantGen later received a Phase II contract award from the NCI [HHSN261201500044C], and a Phase II grant from the NIDA [4R44DA035531-02] to develop rabbit monoclonal antibodies against an additional 100 cancer-related proteins and more than 100 neuroscience related proteins, respectively. We have made the developed research antibodies available to the scientific community and will continue to add to the list as they are validated.

 

New Research Antibodies

 

Current Research Antibodies

Catalog No Protein/Gene UniProt # Data Sheet
RA-1001 ANK1 P16157 Download PDF Request Quote
RA-1002 ANXA1 P04083 Download PDF Request Quote
RA-1003 ANXA3 P12429 Download PDF Request Quote
RA-1004 ANXA5 P08758 Download PDF Request Quote
RA-1005 ENOA/ENO1 P06733 Download PDF Request Quote
RA-1006 EPHB2 P29323 Download PDF Request Quote
RA-1007 MAPK3 P27361 Download PDF Request Quote
RA-1008 AKT1 P31749 Download PDF Request Quote
RA-1009 Vimentin P08670 Download PDF Request Quote
RA-1010 ALPL P05186 Download PDF Request Quote
RA-1011 ICAM1 P05363 Download PDF Request Quote
RA-1012 LNPEP Q9UIQ6 Download PDF Request Quote
RA-1013 Cadherin-6 P06733 Download PDF Request Quote
RA-1014 Gnb2 P62880 Download PDF Request Quote
RA-1015 Gnb3 Q61011 Download PDF Request Quote
RA-1016 Gabrb2 P63137 Download PDF Request Quote
RA-1017 Pkrcb (phospho T377) P68404 Download PDF Request Quote
RA-1018 Gria1 P23818 Download PDF Request Quote
RA-1019 Cnr1 P47746 Download PDF Request Quote
RA-1020 Gria3 Q9Z2W9 Download PDF Request Quote
RA-1021 Snap47 Q8R570 Download PDF Request Quote

 

Rabbit Monoclonal Antibodies for Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) Mass Spectrometry Studies

Dr. Leigh Anderson introduced the term SISCAPA, or Stable Isotope Standard Capture with Anti-Peptide Antibodies. SISCAPA is a hybrid method, combining immunoprecipitation (IP) and stable isotope dilution multiple reaction monitoring (SID-MRM) mass spectrometer to isolate and quantify proteins and to measure biomarker candidates directly from plasma. We have validated all of the antibodies on the above list western blot analysis and immunoprecipitation assay followed by Mass Spectrometer analysis of the immunoprecipitated peptides.  We have not tested these antibodies in immunohistochemistry, immunocytochemistry, or immunofluorescence.