The rabbit monoclonal antibody advantage

The rabbit immune system generates and affinity matures antibodies by mechanisms that differ from those of mice and other rodents. Antibodies produced by rabbits normally have 10 to 100-fold higher affinity for antigen than mouse monoclonal antibodies. In addition, the rabbit immune system is particularly adept at generating antibodies that are able to distinguish between very similar molecules with subtle structural variations.  Thus, these products are exceptional research antibodies.

In 2015, the NCI awarded a contract to AvantGen to develop rabbit monoclonal antibodies against peptides derived from potential cancer biomarkers. Based on the high quality of the anti-peptide antibody clones that were generated from Phase I projects, AvantGen later received a Phase II contract award from the NCI [HHSN261201500044C], and a Phase II grant from the NIDA [4R44DA035531-02] to develop rabbit monoclonal antibodies against an additional 100 cancer-related proteins and more than 100 neuroscience related proteins, respectively. We have made the developed research antibodies available to the scientific community and will continue to add to the list as they are validated.

 

New Research Antibodies

 

Current Research Antibodies

Catalog No Protein/Gene UniProt # Data Sheet
RA-1001 (ANK1) P16157 Download PDF Request Quote
RA-1002 (ANXA1) P04083 Download PDF Request Quote
RA-1003 (ANXA3) P12429 Download PDF Request Quote
RA-1004 (ANXA5) P08758 Download PDF Request Quote
RA-1005 (ENOA/ENO1) P06733 Download PDF Request Quote
RA-1006 (EPHB2) P29323 Download PDF Request Quote
RA-1007 (MAPK3) P27361 Download PDF Request Quote
RA-1008 (AKT1) P31749 Download PDF Request Quote

 

Rabbit Monoclonal Antibodies for Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) Mass Spectrometry Studies

Dr. Leigh Anderson introduced the term SISCAPA, or Stable Isotope Standard Capture with Anti-Peptide Antibodies. SISCAPA is a hybrid method, combining immunoprecipitation (IP) and stable isotope dilution multiple reaction monitoring (SID-MRM) mass spectrometer to isolate and quantify proteins and to measure biomarker candidates directly from plasma. All of the antibodies on the above list have been validated in western blot analysis and immunoprecipitation assay followed by Mass Spectrometer analysis of the immunoprecipitated peptides.  These antibodies have not been tested in immunohistochemistry, immunocytochemistry, or immunofluorescence.