The rabbit monoclonal antibody advantage
The rabbit immune system generates and affinity matures antibodies by mechanisms that differ from those of mice and other rodents. Antibodies produced by rabbits normally have 10 to 100-fold higher affinity for antigen than mouse monoclonal antibodies. In addition, the rabbit immune system is particularly adept at generating antibodies that are able to distinguish between very similar molecules with subtle structural variations. Thus, these products are exceptional research antibodies.
In 2015, the NCI awarded a contract to AvantGen to develop rabbit monoclonal antibodies against peptides derived from potential cancer biomarkers. Based on the high quality of the anti-peptide antibody clones that were generated from Phase I projects, AvantGen later received a Phase II contract award from the NCI [HHSN261201500044C], and a Phase II grant from the NIDA [4R44DA035531-02] to develop rabbit monoclonal antibodies against an additional 100 cancer-related proteins and more than 100 neuroscience related proteins, respectively. We have made the developed research antibodies available to the scientific community and will continue to add to the list as they are validated.
Current Research Antibodies
Rabbit Monoclonal Antibodies for Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) Mass Spectrometry Studies
Dr. Leigh Anderson introduced the term SISCAPA, or Stable Isotope Standard Capture with Anti-Peptide Antibodies. SISCAPA is a hybrid method, combining immunoprecipitation (IP) and stable isotope dilution multiple reaction monitoring (SID-MRM) mass spectrometer to isolate and quantify proteins and to measure biomarker candidates directly from plasma. We have validated all of the antibodies on the above list western blot analysis and immunoprecipitation assay followed by Mass Spectrometer analysis of the immunoprecipitated peptides. We have not tested these antibodies in immunohistochemistry, immunocytochemistry, or immunofluorescence.