AvantGen’s proprietary yeast display system is a highly robust display system based on a novel synthetic anchoring peptide. Although phage display has been extensively used for protein engineering, including antibody discovery and optimization, it has been well documented that phage display is highly biased in its ability to display antibodies derived from different human antibody germline variable region genes, or even antibody clones that differ with only a single point mutation. AvantGen’s display system, in contrast, shows no such bias. Shown below is the comparison of phage display and AvantGen’s yeast display of human antibodies. AvantGen’s innovative display system is used for the discovery of both novel human and rabbit monoclonal antibodies, as well as for antibody humanization and optimization.
AvantGen’s yeast display system is well suited for displaying rabbit antibodies whose light chains normally have an intra-light chain disulfide bond that is required for maintaining their high affinity and specificity.
AvantGen Yeast Display
AvantGen’s yeast display is highly uniform: < 2 fold difference in antibody display levels of all different human VH/VL pairs tested
Phage Display
Prokaryotic expression results in > 30 fold difference in antibody display between different variable region families (Morphosys; mAbs 5:3, 1-25; 2013)