AvantGen has constructed 30 human antibody libraries (Germliner™) with multiple human antibody germline frameworks. These were selected based on their high stability, solubility and expression level. Diversity is focused in the CDR regions and mimics the diversity of the natural human antibody repertoire for that variable region family. The framework sequences are left as fully human germline sequences. AvantGen’s proprietary libraries are comprised of >100 billion members, are well expressed and displayed with AvantGen’s yeast display system allowing efficient human antibody discovery.
Coupled with AvantGen’s proprietary screening technologies, we can rapidly isolate fully human antibodies against not only the antigen, but also the epitope of interest and with other desired characteristics, such as high affinity (sub-nM to low nM), high specificity, and functional activity from the libraries within weeks.
Starting with the target of interest, AvantGen can perform a complete antibody discovery service. AvantGen will perform the screening, isolation, identification, and characterization of the novel antigen-positive antibody clones. The VH and Vκ/λ antibody fragments can be readily subcloned into the full-length IgG format of choice.
AvantGen can also produce antigens and develop and run functional assays to support the human antibody discovery program.
The following is an example of human antibody clones isolated from AvantGen antibody libraries. Both display high affinity and functional blocking activity.
Human antibody clones that are able to recognize different isoforms, or different protein conformations, with species-cross reactivity can also be isolated readily from our large human antibody libraries, as shown in the following figure.