Rabbit Monoclonal Antibodies for Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) Mass Spectrometry Studies
SISCAPA, Stable Isotope Standard Capture with Anti-Peptide Antibodies, is a term introduced by Dr. Leigh Anderson. SISCAPA is a hybrid method, combining immunoprecipitation (IP) and stable isotope dilution multiple reaction monitoring (SID-MRM) mass spectrometer to isolate and quantify proteins and to measure biomarker candidates directly from plasma. AvantGen has been awarded a NCI contract to develop rabbit monoclonal antibodies against peptides derived from potential cancer biomarkers. Antibodies generated through this contract are now available for the research community.
|Catalog No||Protein/Gene||UniProt #||Data Sheet|
|RA-1001||(ANK1)||P16157||Download PDF||Request Quote|
|RA-1002||(ANXA1)||P04083||Download PDF||Request Quote|
|RA-1003||(ANXA3)||P12429||Download PDF||Request Quote|
|RA-1004||(ANXA5)||P08758||Download PDF||Request Quote|
|RA-1005||(ENOA/ENO1)||P06733||Download PDF||Request Quote|
|RA-1006||(EPHB2)||P29323||Download PDF||Request Quote|
|RA-1007||(MAPK3)||028482||Download PDF||Request Quote|
|RA-1008||(AKT1)||P31749||Download PDF||Request Quote|
All of the antibodies on the list have been validated in immunoprecipiation assay followed by Mass Spectrometer analysis of the immunoprecipitated peptides.
Based on the high quality of the anti-peptide antibody clones that were generated from Phase I projects, AvantGen also recently received a Phase II contract award from the NCI [HHSN261201500044C], and a Phase II grant from the NIDA [4R44DA035531-02] to develop rabbit monoclonal antibodies against an additional 100 cancer-related proteins and more than 100 neuroscience related proteins, respectively. The developed antibodies will be made available to the research community and added to the list as they are validated.